STIC- Members: Isabella Derler

An Overview

Curriculum Vitae

Post Doc

Institut für Biophysik
Johannes Kepler Universität Linz
Altenbergerstraße 69
A-4040 Linz
Austria

Phone:+4373224689274
Fax:+4373224689280
Email: isabella.derler@jku.at

Born:

10.3.1983 in Linz, Austria

Education:

September 1989 – July 1994: Elementary School: VS Auwiesen
September 1994 – June 2001: Grammar School: Kreuzschwestern Linz (Realgymnasium)
August 2001: Diplom Supérieur d’Etudes Françaises Modernes (Alliance Française; Cannes)
October 2001 – July 2005: Diploma studies of Technical Physics (University of Linz)
August 2005 – July 2008: PhD at Institute for Biophysics (University of Linz)
August 2008 – : Post-Doc at the Institute for Biophysics (University of Linz)

Scholarship:

  • Scholarship for PhD thesis 2006 of the Austrian academy of science for 24 months (STIM: the missing link to store-operated channel activation of TRP channels?)
  • Scholarship for 1 year of Post-Doc career - Habilitationsstipendium 2009 - by JKU goes Gender (Disease-related STIM1 and ORAI1 mutants affecting store-operated currents)

Awards:

Wilhelm-Macke-Award 2005 for diploma thesis: Molecular mechanism of TRP channel regulation

Junior Research Award – GEN-AU Summer School Litschau 2008 for the talk: Increased hydrophobicity at the N-terminus/membrane interface impairs ORAI1 SCID mutant gating

Award of Excellence 2008 for PhD thesis: Dynamic regulation of Ca2+ channels by Ca2+ binding proteins

Teaching Activities:

Cell culture lecture and practical course (SS 2009)

Meeting Abstracts:

Talk:

  • Litschau, Austria; GEN-AU Summer School; September 17-19; 2008
    I. Derler, M. Fahrner, O. Carugo, M. Muik, J. Bergsmann, R. Schindl, I. Frischauf, S. Eshaghi and C. Romanin.
    Increased hydrophobicity at the N-terminus/membrane interface impairs ORAI1 SCID mutant gating
  • Long Beach, California, USA; Biophysical Society; February 3 – 7; 2008
    I. Derler, M. Muik, I.Frischauf, M. Fahrner, B. Polzinger, R.Schindl, J. Bergsmann, R. Fritsch, H. Kahr, J. Madl, K. Groschner and C. Romanin
    Dynamic physical coupling of STIM1 and ORAI1 is mediated by the C-terminus of ORAI1 rather than by its N-terminus. Biophy. Soc. 2007 1824-Plat.
  • Department for medical/pharmaceutical chemistry at the University Vienna, 25.01.2008
    Presentation (“Impulsreferat”) about “Dynamic coupling of STIM1 to ORAI1” the work carried out during this scholarship (project entitled: STIM: the missing link to store-operated activation of TRP channels?), Workshop A for “Bio-Science and Medicine” chaired by Prof. Christian Noe

Posters:

  • Lucca (Barga), Italy: Gordon Research Conference, June 21 - 26, 2009
    Isabella Derler*, Marc Fahrner*, Martin Muik*, Barbara Lackner, Rainer Schindl, Klaus Groschner and Christoph Romanin.
    A CRAC modulatory domain (CMD) within STIM1 mediates fast Ca2+-dependent inactivation of ORAI1 channels.
  • Boston, Massachusetts, USA; Biophysical Society Meeting, February 28 - March 4, 2009
    I. Derler, M. Fahrner, O. Carugo, M. Muik, J. Bergsmann, R. Schindl, I. Frischauf, S. Eshaghi and C. Romanin.
    Increased hydrophobicity at the N-terminus/membrane interface impairs ORAI1 SCID mutant gating; Biophy. Soc. 2009 B594
  • Homburg, Germany; Spatio-temporal interactions of cellular signalling molecules; Mai 18-19, 2007
    M. Muik*, I.Frischauf*, I. Derler*, M. Fahrner, B. Polzinger, R.Schindl, J. Bergsmann, R. Fritsch, H. Kahr, J. Madl, K. Groschner, C. Romanin
    *These authors contributed equally.
    Visualization of dynamic physical interaction of STIM1 and ORAI1
    P23
  • Baltimore, Maryland, USA; Biophysical Society Meeting; March 3-7, 2007
    I. Derler, R. Schindl, H. Kahr, M. Muik, R. Fritsch, V. Lehen’kyi, N. Prevarskaya, M. Hediger, K. Groschner, C. Romanin
    Molecular components contributing to store-operated current (SOC) in prostate cancer cell line LNCaP; Biophy. Soc. 2007
    B182
  • Bath, England; Cell and Molecular Biology of TRP channels; September 7-8, 2006
    Isabella Derler, Rainer Schindl, Heike Kahr, Petra Eder, Matthias Hediger, Klaus Groschner and Christoph Romanin
    Partial reconstitution of store-operated current (SOC) by TRPV6 in LNCaP prostate carcionoma cells - Evidence for a BTP2 sensitive component fully restoring endogenous SOC.
    Biochem. Soc., P16
  • München, Germany; Deutsche Physiologische Gesellschaft; March 28, 2006
    Isabella Derler, Michael Hofbauer, Heike Kahr, Reinhard Fritsch, Martin Muik, Klaus Kepplinger, Marlene Hack, Sieglinde Moritz, Rainer Schindl and Christoph Romanin
    Dynamic Ca2+-dependent association of calmodulin with the C-terminus of TRPV6 channel correlates with its inactivation.
    PT03P-10
  • Leuven, Belgium; TRP channels: Unique players in cell function; September 15 – 17, 2005
    Isabella Derler, Michael Hofbauer, Heike Kahr, Reinhard Fritsch, Martin Muik, Klaus Kepplinger, Marlene Hack, Sieglinde Moritz, Rainer Schindl and Christoph Romanin
    Dynamic Ca2+-dependent association of calmodulin with the C-terminus of TRPV6 channel correlates with its inactivation.
    Abstract # 9

Publications:

  1. Schindl R, Frischauf I, Bergsmann J, Muik M, Derler I, Lackner B, Groschner K, Romanin C.
    Plasticity in Ca2+ selectivity of Orai1/Orai3 heteromeric channel. Proc Natl Acad Sci U S A. 2009 Nov 3
  2. Fahrner M, Muik M, Derler I, Schindl R, Fritsch R, Frischauf I, Romanin C.
    Mechanistic view on domains mediating STIM1-Orai coupling. Immunol Rev. 2009 Sep;231(1):99-112.
  3. Schindl R, Muik M, Fahrner M, Derler I, Fritsch R, Bergsmann J, Romanin C.
    Recent progress on STIM1 domains controlling Orai activation. Cell Calcium. 2009 Oct;46(4):227-32.
  4. Isabella Derler*, Marc Fahrner*, Martin Muik*, Barbara Lackner, Rainer Schindl, Klaus Groschner and Christoph Romanin.
    * These three authors contributed equally.
    A CRAC modulatory domain (CMD) within STIM1 mediates fast Ca2+-dependent inactivation of ORAI1 channels. J Biol Chem. 2009 Sep 11;284(37):24933-8.
  5. Irene Frischauf*, Martin Muik*, Isabella Derler*, Judith Bergsmann, Marc Fahrner, Rainer Schindl, Klaus Groschner, Christoph Romanin.
    * These three authors contributed equally.
    Coiled-coil mediated coupling between STIM1 and ORAI channels: Differential activation of ORAI1,2,3 channels by a STIM1 mutant. J Biol Chem. 2009 Aug 7;284(32):21696-706.
  6. Isabella Derler*, Marc Fahrner*, Oliviero Carugo, Martin Muik, Judith Bergsmann, Rainer Schindl, Irene Frischauf, Said Eshaghi and Christoph Romanin.
    * These two authors contributed equally
    Increased hydrophobicity at the N-terminus/membrane interface impairs gating of the SCID-related ORAI1 mutant. J Biol Chem. 2009 Jun 5;284(23):15903-15.
  7. Martin Muik*, Marc Fahrner*, Isabella Derler*, Rainer Schindl, Judith Bergsmann, Irene Frischauf, Klaus Groschner, Christoph Romanin
    * These three authors contributed equally.
    A cytosolic homomerization and a modulatory domain within STIM1 C-terminus determine coupling to ORAI1: Identification of a minimal STIM1 fragments that confers maximal activation of ORAI1 currents. JBC 2009, Mar 27;284(13):8421-6
  8. Frischauf I, Schindl R, Derler I, Bergsmann J, Fahrner M, Romanin C.
    The STIM/Orai coupling machinery. Channels (Austin). 2008 Jul-Aug; 2(4):261-8.
  9. Isabella Derler*, Reinhard Fritsch, Rainer Schindl, Christoph Romanin*
    *Author for correspondence
    CRAC inhibitors: identification and potential; Expert Opinion on Drug Discovery, July 2008, Vol. 3, No. 7, Pages 787-800.
  10. Rainer Schindl*, Judith Bergsmann*, Irene Frischauf, Isabella Derler, Marc Fahrner, Martin Muik, Reinhard Fritsch, Klaus Groschner, Christoph Romanin.
    *These two authors contributed equally.
    2-Aminoethoxydiphenyl borate alters selectivity of ORAI3 channels by increasing their pore size; J Biol Chem. 2008 Jul 18;283(29):20261-7. Impact Factor: 5,8
  11. M. Muik*, I.Frischauf*, I. Derler*, M. Fahrner, Bergsmann J, Eder P, Schindl R, Hesch C, Polzinger B, Fritsch R, Kahr H, Madl J, Gruber H, Groschner K, Romanin C.
    *These authors contributed equally.
    Dynamic physical coupling of the putative coiled-coil domain of ORAI1 with STIM1 mediates ORAI1 channel activation. J Biol Chem. 2008; 283(12):8014-22; Impact Factor: 5,8
    Mentioned as Research Highlight in Nature: Come together; Vol 451; 503
  12. R. Schindl*, I. Frischauf*, H. Kahr, R. Fritsch, M. Krenn, A. Derndl, E. Vales, M. Muik, I. Derler, K. Groschner and C. Romanin
    *These authors contributed equally.
    The first ankyrin-like repeat is the minimum indispensable key structure for functional assembly of homo and heteromeric TRPC4/TRPC5 channels. Cell Calcium. 2008; 43(3):260-9; Impact Factor: 4,1
  13. Isabella Derler*, Michael Hofbauer*, Heike Kahr, Reinhard Fritsch, Martin Muik, Klaus Kepplinger, Marlene Hack, Sieglinde Moritz, Rainer Schindl and Christoph Romanin.
    *These authors contributed equally.
    Dynamic but not constitutive association of calmodulin with rat TRPV6 channels enables fine tuning of Ca2+-dependent inactivation. J. Pysiol. 2006; 577(Pt 1):31-44. Impact Factor: 4,4.
  14. Michael Poteser*, Annarita Graziani*, Christian Rosker, Petra Eder, Isabella Derler, Michael X. Zhu, Christoph Romanin and Klaus Groschner.
    *These authors contributed equally.
    TRPC3 and TRPC4 associate to form a redox-sensitive cation channel - Evidence for expression of native TRPC3/ TRPC4 heteromeric channels in endothelial cells. J Biol Chem., 2006, 281(19):13588-95. Impact Factor: 5,8.

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